| Catalog No. | Product Name | Specification |
| CRH00-3061 | ResiQuant® HEK 293 HCP ELISA Kit | 48T |
| CRH00-3062 | ResiQuant® HEK 293 HCP ELISA Kit | 96T |
Product Overview
The ResiQuant® HEK 293 HCP ELISA Kit is a simple-to-use and quantitative ELISA kit. This kit is specifically intended for the detection of host cell protein contaminants in products derived from human embryonic kidney 293 (HEK 293) expression. The limit of detection (LOD) for this kit is 5 ng/mL, and the limit of quantitation (LLOQ) is 10 ng/mL. It is suitable for use throughout the manufacturing process as well as for product release.
● Sensitive measurement: the limit of detection (LOD) is 5 ng/ml and the limit of quantification (LLOQ) is 10 ng/ml; spiking recovery rate qualified, within the range of 70%-130%
● High Antibody Coverage: Characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, with 88% coverage, highly risk impurities also included
Specifications & Data
| LOD | 5 ng/ml | Storage | 2°C to 8°C |
| LLOQ | 10 ng/mL | Shipping Condition | Dry Ice |
| Recovery Rate | 70%~130% | Host Cell | HEK 293 |
| Intra-batch | ≤15% | Shelf Life | 12 months |
| Inter-batch | ≤20% | Quantity | 48/96 reactions |
| Accuracy | RSD≤20% | Includes | Assay Kit only |
Fig 1. Data analysis is suggested using a nonlinear fit of the sigmoidal curve generated by a range of standard concentrations. |
Fig 2. Antibody coverage rate was evaluated by AAE and LC-MS. HEK 293 HCPs and AAE purified HEK 293 HCPs were applied to LC-MS as pre-AAE and Post-AAE respectively. The sum of the identified post-AAE and pre-AAE HCPs was recognized as the total number of the whole HCPs. The recovery rate was calculated using the number of the whole HCPs divided by the number of post-AAE HCPs. |
Documents
Manual_CRH306X
MSDS_CRH306X
Application
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